Bacterial cells having a glyoxylate shunt for the manufacture of succinic acid

摘要

The present invention is concerned with bacteria for the production of succinic acid. Specifically, the invention relates to a bacterial cell of the genus Pasteurella comprising a heterologous polypeptide having isocitrate lyase activity and a heterologous polypeptide having malate synthase activity. Further, the present invention contemplates a polynucleotide comprising a nucleic acid encoding a polypeptide having isocitrate lyase activity and a nucleic acid encoding a polypeptide having malate synthase activity. Finally, the present invention relates to the use of a bacterial cell of the invention for the manufacture of succinic acid.

主权项

1. A bacterial cell of the genus Pasteurella comprising a heterologous polypeptide having isocitrate lyase activity and a heterologous polypeptide having malate synthase activity, wherein the bacterial cell has increased succinic acid production relative to a corresponding control bacterial cell,
wherein the heterologous polypeptide having isocitrate lyase activity is encoded by a nucleic acid selected from the group consisting of: a. a nucleic acid having the nucleotide sequence of SEQ ID NO: 1; b. a nucleic acid encoding the amino acid sequence of SEQ ID NO: 2; c. a nucleic acid which is at least 98% identical to the nucleic acid of a) or b); and d. a nucleic acid encoding a polypeptide comprising an amino acid sequence which is at least 98% identical to the amino acid sequence encoded by the nucleic acid of a) or b), and wherein the heterologous polypeptide having malate synthase activity is encoded by a nucleic acid selected from the group consisting of: a. a nucleic acid having the nucleotide sequence of SEQ ID NO: 3; b. a nucleic acid encoding the amino acid sequence of SEQ ID NO: 4; c. a nucleic acid which is at least 98% identical to the nucleic acid of a) or b); and d. a nucleic acid encoding a polypeptide comprising an amino acid sequence which is at least 98% identical to the amino acid sequence encoded by the nucleic acid of a) or b).