Methods for identification of tumor phenotype and treatment

摘要

The disclosure relates to methods for identifying a tumor as an E2F-responsive gene over-expressing (ERGO) tumor, methods of determining the likelihood that an ERGO tumor patient will survive to a future date, methods of treating an ERGO tumor in a patient, and methods of selecting patients diagnosed as ERGO tumor prostate cancer patients for aggressive clinical treatment. The methods of the disclosure are applicable to ERGO tumors present in different human organs and tissues such as breast, lung, thyroid, ovary, and prostate.

主权项

1. A method of identifying a tumor as an ERGO tumor comprising the steps of:
a) providing a tumor sample; b) providing a reference from non-cancerous tissue; c) providing a nucleic acid array comprising at least 21 of the nucleic acid sequences shown in SEQ ID NO:s 12-14, 20-21, 35, 37, 39, 43-44, 46-47, 49-51, 54, 56, 58-72, 75, 79-84, 86, 88, 91.93-95, 102-105, 107-110, 112, 116, 118-120, 122-123, 125-126, 128-129, 131, 145-146, 148-149, 151, 154, 156-157, 160, 163-166, 169, 171, 173-174, 176-177, 180, 184, 186, 188, 193, 196, 198, 200-201, 203, 205, 209, 217, 246-247, 249, 254, 259, 267, 269, 274-275, 292-295, 298, 307, 311, 316, 321-323, 331, 333, and 335-340 adapted for hybridization to nucleic acids in a tumor sample; d) measuring an indicator of gene transcript levels in the tumor sample to produce an indicator value for each of the following gene transcripts having the nucleic acid sequences shown in SEQ ID NO:s 12-14, 20-21, 35, 37, 39, 43-44, 46-47, 49-51, 54, 56, 58-72, 75, 79-84, 86, 88, 91, 93-95, 102-105, 107-110, 112, 116, 118-120, 122-123, 125-126, 128-129, 131, 145-146, 148-149, 151, 154, 156-157, 160, 163-166, 169, 171, 173-174, 176-177, 180, 184, 186, 188, 193, 196, 198, 200-201, 203, 205, 209, 217, 246-247, 249, 254, 259, 267, 269, 274-275, 292-295, 298, 307, 311, 316, 321-323, 331, 333, and 335-340 by hybridizing a nucleic acid obtained from the tumor sample to the nucleic acid array: e) measuring the reference to produce a reference value; and f) comparing the indicator value to the reference value to determine which of the gene transcripts is over-expressed;whereby the tumor is identified as an ERGO tumor if at least 21 of these gene transcripts are over-expressed.