Marker for identifying variety/line of plant of the genus <i>Saccharum </i>and the use thereof

摘要

It is an object of the present invention to provide a method for identifying the variety/line of a plant of the genus Saccharum with the use of novel DNA markers that allow high-precision identification of a wide range of varieties/lines of plants of the genus Saccharum. A method for identifying the variety/line of a plant of the genus Saccharum, comprising using a simple sequence repeat polymorphism in at least one DNA sequence selected from SEQ ID NOS: 1 to 12 is provided.

主权项

1. A method for identifying the variety/line of a plant of the genus Saccharum, comprising a step of genotyping at least one simple sequence repeat selected from simple sequence repeat polymorphisms in DNA sequences of SEQ ID NOs: 1, 2, 3, 5, 6, 10, and 12, wherein the step of genotyping is carried out by the steps of:
(a) carrying out PCR amplification using DNA extracted from a sugarcane to be identified as a template and a primer set consisting of a forward and a reverse primer that specifically amplify a region containing a simple sequence repeat in a DNA sequence of SEQ ID NOs: 1, 2, 3, 5, 6, 10, or 12; (b) determining the molecular weights of amplified DNA fragments; and (c) performing genotyping of the region containing a simple sequence repeat based on distribution of molecular weights.