Fast results hybrid capture assay on an automated platform

摘要

The present invention comprises a method that provides fast and reliable results for detecting the presence of a target nucleic acid molecule in a sample.

主权项

1. A method for determining the presence of a target nucleic acid molecule in a sample, the method comprising:
a) suspending the sample in a collection medium comprising an anionic detergent and a non-ionic detergent; b) denaturing the target nucleic acid molecule in the sample; c) forming a double-stranded nucleic acid hybrid by contacting at least one polynucleotide probe with the target nucleic acid molecule; d) forming a double-stranded nucleic acid hybrid-support complex by capturing the double-stranded nucleic acid hybrid on a magnetic bead comprising a first antibody; e) forming a double-stranded nucleic acid hybrid-support-second antibody complex by contacting the double-stranded nucleic acid hybrid-support complex with a second antibody, wherein the second antibody is labeled with a detectable marker; f) washing the double-stranded nucleic acid hybrid-support-second antibody complex with a wash buffer; and g) detecting the marker on the second antibody wherein the detecting indicates the presence of the target nucleic acid molecule,wherein the collection medium is present at least from step a) to step d).