Thermally induced gelation of collagen hydrogel and method of thermally inducing gelling a collagen hydrogel

摘要

The present invention relates to collagen hydrogels. Particularly, the invention relates to hydrogels comprising a telopeptide collagen (“telo-collagen”) and an atelopeptide collagen (“atelo-collagen”); hydrogels comprising collagen and chitosan; methods of making the hydrogels; methods of reducing gelation of a hydrogel mixture at room temperature; methods of reducing compaction of cells; and methods of culturing cells on such hydrogels.

主权项

1. A method of hastening gelation at room temperature of a telopeptide containing hydrogel comprising: providing a telopeptide-collagen; adding an atelopeptide-collagen thereby forming a collagen mixture wherein the telopeptide-collagen is 5-25 weight percent of total collagen and wherein the atelopeptide-collagen represents 75-95 weight percent of the total collagen; storing the mixture at a temperature that is less than about 37° C., wherein the mixture has hastened gelation.