Systems, devices, and methods for measuring whole blood hematocrit based on initial fill velocity

摘要

Methods for determining the hematocrit of a blood sample, and devices and systems used in conjunction with the same. The hematocrit value can be determined on its own, and further, it can be further used to determine a concentration of an analyte in a sample. In one exemplary embodiment of a method for determining the hematocrit value in a blood sample, a volume of blood is provided in a sample analyzing device having a working and a counter electrode. An electric potential is applied between the electrodes and an initial fill velocity of the sample into the device is calculated. The hematocrit of the blood, as well as a concentration of an analyte in view of the initial fill velocity can then be determined. Systems and devices that take advantage of the use of an initial fill velocity to determine hematocrit levels and make analyte concentration determinations are also provided.

主权项

1. A method for determining an analyte of a whole blood sample, the method comprising the steps of:
providing a sample of whole blood to a sample analyzing device having a capillary space; measuring an initial fill velocity of the sample in at least a portion of the capillary space in which the initial velocity is measured by:
applying an electrical potential;measuring an electrical current; anddetermining an initial current flow; determining a hematocrit value of the blood sample solely from the measured initial fill velocity using an equation that requires the determined initial current flow and wherein the sample includes an analyte, and calculating a concentration of the analyte by:
applying an electric potential;measuring an initial current after applying the electric potential;reversing the electric potential;measuring a change in current over time following the reversal of the electric potential as a slope (m) of a current versus time graph; andusing an equation that requires the determined hematocrit value and the measured change in current over time.