| 摘要 |
Provided are methods and processes for producing glycoproteins by eukaryotic cell culture which provide a glycoprotein product containing oligosaccharides terminating in one or more sialic residues by inhibiting sialidase expression and/or activity in the cell culture. Sialidase expression and/or activity was shown to be inhibited by the addition of a trace element, e.g. zinc or cobalt, or insulin-like growth factor 1 (IGF-1) to the cell culture medium. |
