摘要 |
<p>Disclosed is a method of producing alcohol from cellulosic and/or lignocellulosic materials, wherein the method comprises the steps of: (a) irradiating a biomass before adding water to the biomass with an electron beam device.; (b) adding water to the biomass material to swell the biomass material; (c) cooling the swollen biomass material to or below about 273 degrees Kelvin, wherein cooling is performed in a freeze grinding or freeze milling device; (d) comminuting and/or grinding the cooled swollen biomass; (e) further irradiating the biomass after comminution of the cooled swollen biomass; and (f) contacting the comminuted cooled biomass material with an enzyme to saccharify the cellulose and utilizing a microorganism to produce an alcohol.</p> |