Method for Removing Endotoxin from Proteins

摘要

Disclosed is a method for removing endotoxin from proteins. Also disclosed are products made by using the method. The method may be used, for example, to produce endotoxin-free lactoferrin. Bovine milk-derived lactoferrin may be produced in commercial quantities by the method, and endotoxin-free bovine lactoferrin may be used for a variety of therapeutic uses, including improving wound healing.

主权项

1. A method for removing endotoxin bound to a protein, the method comprising
a) binding the protein to a cation exchange resin; b) eluting the endotoxin using a low-ionic strength solution in the absence of added surfactant; and c) eluting the protein with a high ionic strength solution.