| 摘要 |
<p>The subject of the present invention is the medical application of N-oleoyl-dopamine and 3'-O-methyl-N-oleoyl-dopamine for the production of a medication for treatment or prophylaxis of disturbances or states due to deficiency of dopamine, preferably chosen from the group covering oxygen deficiencies (hypoxia) in anesthetized and awake mammals, breathing with atmospheric oxygen (normoxia), for the respiratory responses to hypoxia in mammals, lack of dopamine binding to the membrane system in the mammalian brain, disturbances in metabolic pathways of dopamine metabolism, states of dopamine deficiencies provoked by morbid or genetic factors as well as under condition connected to the physiological process of aging of the organism, through the systemic introduction of the compound to the mammalian organism. The subject of the present invention is also the method of production of 3'-O-methyl-N-oleoyl-dopamine in vitro which consists of, according to the present invention, a reaction of 3-O-methyl-dopamine hydrochloride with oleic acid in the presence of, preferably, BOP
(Benzotriazole-1-yl-oxy-tris-(dimethylamino)-phosphonium hexafluorophosphate), DCC ( N , N '-dicyclohexylcarbodiimide), DMAP (4-Dimethylaminopyridine) and THF (tetrahydrofuran), CH 2 Cl 2 (dichloromethane), CHCl 3 (chloroform), toluene, diethyl ether. The reaction mixture is cooled to temperature of 0-10°C, a solution of trietanolamine is added under continuous stirring and
3'-O-methyl-N-oleoyl-dopamine is precipitated and purified by a known method. A variant of the method of production of 3'-O-methyl-N-oleoyl-dopamie consists of, according to the present invention, a reaction of methylation of N-oleoyl-dopamine by the catechol-O-methyltransferase (COMT) in the presence of
S-adenosyl-L-methionine (SAM) and magnesium ions in phosphate buffered saline (PBS) solution buffered to pH of 7.0-8.0 at temperature of 25-45°C, followed by the precipitation and purification of 3'-O-methyl-N-oleoyl-dopamine by a known method.</p> |
