NOVEL PRONGF MUTANTS AND USES THEREOF IN THE PRODUCTION OF BETA-NGF
摘要
The present invention relates to a proNGF mutant and to uses thereof, in particular the use of a proNGF mutant for producing human beta-NGF. The present invention discloses a method of preparing a biologically active human beta-NGF from an inactive insoluble proNGF mutant. A proNGF mutant of the invention is substituted by any amino acid but not Arg or Lys at the native protease cleavage site R 1 SK 3 R 4 at least at positions R 1 and K 3 corresponding to positions 101 and 103 of the human wildtype proNGF sequence.
申请公布号
SG11201402585P(A)
申请公布日期
2014.10.30
申请号
SG11201402585P
申请日期
2012.12.19
申请人
WACKER CHEMIE AG
发明人
LOREY, SUSAN;JANOWSKI, BERNHARD;PULTKE, HEIKO;KATHMANN, DANIELA;PARTHIER, ANTJE;ANTON, ANDREAS