| 摘要 |
PROBLEM TO BE SOLVED: To provide iPS progenitor cells capable of efficient gene transfer by being capable of stably maintaining cultures.SOLUTION: iPS progenitor cells are produced by introducing any one of OCT4, SOX2, and KLF4 into human somatic cells and culturing them in a culture medium containing a MEK inhibitor, a GSK3&bgr; inhibitor, and a leukaemia inhibitor factor (LIF). The MEK inhibitor is, for example, PD0325901. The GSK3&bgr; inhibitor is, for example, CHIR99021. The iPS progenitor cells are OCT4-negative, NANOG-negative, and NESTIN-positive. |
