System and Method to Improve Yield of Sorted Particles

摘要

Provided herein are systems and methods for improving yield of sorted particles. In one embodiment, for example, there is provided a system including: (a) a flow cytometer to analyze a sample, wherein the flow cytometer provides a parameter plot based on the analysis of the sample; (b) a user-interface, wherein a user can define a coincidence acceptance gate in the parameter plot, and wherein the coincidence acceptance gate identities a non-target particle population in the sample that may be accepted with a target particle in a subsequent sort analysis; and (c) a sort analysis system to sort particles within the sample, while accepting particles defined by coincidence acceptance gate.

主权项

1. A method of sorting particles within a sample, with use of a flow cytometer, the method comprising:
(a) analyzing a sample having a plurality of particle populations with a flow cytometer; (b) obtaining a parameter plot from the flow cytometry analysis of step (a); (c) defining a coincidence acceptance gate in the parameter plot of step (b), wherein the coincidence acceptance gate identifies a non-target particle population that may be accepted with a target particle in a subsequent sort analysis; and (d) processing the sample through a sort analysis.