Method to Measure Endogenous Enzymatic Serum/Plasma Cholesterol Esterification by LCAT (Lecithin:Cholesterol Acyltransferase) Assay

摘要

The present invention provides a method for the assessment of cholesterol esterification through physiologically relevant pathways and incorporates the function of individual subject's endogenous HDL particles. This ex vivo approach avoids the use of an artificial substrate and provides for determination of LCAT activity that includes both the contribution of a subject's endogenous HDL function and endogenous LCAT protein, and is therefore a significant improvement to the biologic relevance.

主权项

1. A method of determining the level of LCAT activity in a sample comprising incubating cells with a tracer, adding the sample to the cells, incubating the sample with the cells, and determining the LCAT activity in the sample.