| 摘要 |
The present invention provides haloalkane substrates, and linkers for connecting such substrates to functional elements (e.g., tags, labels, surfaces, etc.). Substrates and linkers described herein find use, for example, in labeling, detection, and immobilization of proteins, cells, and molecules. In particular, the linkers provided herein find use within substrates for dehalogenase variants that form covalent bonds with their haloalkane substrates. |
| 主权项 |
1. A compound of formula R-L1-M-L2-A-X;
wherein R is a functional group, L1 is a first linker portion, M is a carbamate group, L2 is a second linker portion, A is an alkyl group, and X is a halogen; wherein L2-A separates M and X by 6-18 linearly connected atoms; and wherein L1 separates R and M by 2 or more linearly connected atoms. |
