HOMOGENEOUS COMPETITIVE LATERAL FLOW ASSAY

摘要

A patient or animal side method and assay for eliminating the hook effect in the detection of a target analyte such as an acute phase protein in a bodily fluid in which the target analyte comprises a member of a specific binding pair comprising applying the sample to a solid phase carrier material, generating a signal in accordance with downstream movement of the labelled first or second members and the target analyte to bind with the complimentary immobilised first or second members, and detecting the presence of the target analyte in accordance with the signal generated at the complimentary immobilised first or second members.

主权项

1. A method for eliminating the hook effect in the detection of a target analyte in a sample in which the target analyte comprises a member of a specific binding pair comprising:
applying the sample to a solid phase carrier material, the solid phase carrier material having labelled first or second members of the specific binding pair thereon and complimentary immobilised first or second members of the specific binding pair downstream of the labelled first or second members of the specific binding pair, generating a signal at the complimentary immobilised first or second members of the specific binding pair in accordance with downstream movement of the labelled first or second members and the target analyte to bind with the complimentary immobilised first or second members, and detecting the presence of the target analyte in accordance with the signal generated at the complimentary immobilised first or second members.