METHODS OF INCORPORATING AMINO ACID ANALOGUES INTO A PROTEIN

摘要

The present invention provides a novel use of a SPP system for replacing natural amino acid residues with non-naturally occurring amino acids in a protein or peptide, using cell based expression system.

主权项

1. A method of producing a target protein containing the targeted replacement of a natural amino acid with an amino acid analogue within a cell-based expression system, wherein the method comprises
a) presenting a trans-acting factor which substantially inhibits or degrades host cell RNA transcripts in conjunction with an expressed mRNA encoding the target protein which is not susceptible to such degradation; b) preventing substantial incorporation of the respective amino acid analogue into host cellular or non-target proteins; c) preventing substantial de novo biosynthesis of a specific proteinogenic amino acid which is targeted for substitution with an amino acid analogue.