| 摘要 |
<p>The present invention relates to a method for quantifying at a functional group (1) functionalizing a surface (2). First, the method comprising the step of providing at a surface (2) having micrometric dimensions functionalized with at a functional group (1). The method includes a step of contacting the surface (2) with a solution: Said solution comprises at a first reagent (4) at a first concentration and a second reagent (5) at a second concentration, the ratio between the first concentration and the second concentration being predetermined. Said first reagent (4) and second reagent (5) are designed for selectively coupling to a functional group (2) with respective affinities, the ratio of said affinities being predetermined. The first reagent (4) is labelled for emitting fluorescence and the second reagent (5) is un-labelled. Then, the fluorescence of said first reagent (4) on the surface to quantify said functional group (1) therefrom.</p> |
