发明名称 |
METHOD FOR EXTRACTING INSULINS BY IMPROVED AIR-GASSING OF A FOLDING |
摘要 |
<p>The present invention comprises a process for preparing insulin or an insulin derivative with correctly linked cysteine bridges from a precursor of said insulin or insulin derivative, wherein said precursor is subjected to a folding process in the presence of cysteine or cysteine hydrochloride and a chaotropic auxiliary compound. The insulin or insulin derivative with correctly linked cysteine bridges is obtained by enzymic cleavage by means of trypsin or a trypsin-like enzyme and, where appropriate, additionally by means of carboxypeptidase B and subsequent purification on an adsorber resin, which process is carried out at varied pH and temperature ranges.</p> |
申请公布号 |
EP1735343(B1) |
申请公布日期 |
2014.10.29 |
申请号 |
EP20050716149 |
申请日期 |
2005.03.17 |
申请人 |
SANOFI-AVENTIS DEUTSCHLAND GMBH |
发明人 |
RUBROEDER, FRANZ-JOSEF;KELLER, REINHOLD;HERBERT, HEIKE |
分类号 |
C07K14/62 |
主分类号 |
C07K14/62 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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