Enhanced deposition of chromogens

摘要

This disclosure relates to compositions that enhance the deposition of detectable moieties on tissue samples, methods utilizing these compositions and kits including these compositions. The compositions include a deposition enhancer having a formula;
where R1, R2, R3, and R4 are independently selected from aliphatic, aryl, halogen, a heteroatom-containing moiety, and hydrogen; R1 and/or R3 can be bound to R2 to form a fused, aromatic ring system; R5 is selected from a heteroatom-containing moiety; A is selected from, a carbon atom, a heteroatom, other than sulfur, and any combination thereof; n is 1-5, an enzyme, a specific binding moiety and a detectable moiety.

主权项

1. A method for detecting a target in a sample by proximally depositing a detectable moiety, comprising:
contacting the sample with a recognition solution, the recognition solution including a specific binding moiety specific to the target; labeling the specific binding moiety with an enzyme; contacting the sample with a detection solution, the detection solution comprising an enzymatic substrate so that the detectable moiety deposits proximally to the target in the presence of a deposition enhancer having a formulawherein R1, R2, R3, and R4 are independently selected from aliphatic, aryl, halogen, a heteroatom-containing moiety, and hydrogen; R1 and/or R3 can be bound to R2 to form a fused, aromatic ring system; R5 is a heteroatom-containing moiety; A is selected from a carbon atom, a heteroatom other than sulfur, and any combination thereof; n is 1-5; and
detecting the detectable moiety.