Method for identifying bacteria from the <i>Bacillus cereus </i>group

摘要

The present invention relates to a method for identifying bacteria of the Bacillus cereus group, comprising the following steps: (a) providing a sample that may contain bacteria of the Bacillus cereus group, a reaction medium comprising at least one fluorescent phosphatidylcholine phospholipase C (PC-PLC) substrate and an inhibitor of Gram-negative bacteria; (b) inoculating the reaction medium with the sample; (c) incubating the inoculated reaction medium; and (d) identifying the bacteria of the Bacillus cereus group by detecting the PC-PLC substrate hydrolysis reaction, in which the pH of the reaction medium and the time necessary for detecting the PC-PLC substrate hydrolysis reaction are adapted such that said hydrolysis reaction by bacteria of the Bacillus cereus group is detected before hydrolysis of the PC-PLC substrate by any Gram-positive bacteria other than those belonging to the Bacillus cereus group, that may be present in the sample.

主权项

1. A method for identifying bacteria of the Bacillus cereus group, comprising the following steps:
(a) providing a sample containing bacteria of the Bacillus cereus group and Gram-positive bacteria other than those belonging to the Bacillus cereus group, a reaction medium comprising at least one fluorescent phosphatidylcholine phospholipase C (PC-PLC) substrate and an inhibitor of Gram-negative bacteria, wherein said reaction medium does not contain an inhibitor of Gram-positive bacteria, and wherein the pH of the reaction medium is between 6.8 and 8.0; (b) inoculating the reaction medium with said sample; (c) incubating the inoculated reaction medium; and (d) identifying the bacteria of the Bacillus cereus group by detecting hydrolysis of the PC-PLC substrate, wherein detection of the hydrolyzed PC-PLC substrate at between 6 and 30 hours results in identification of said bacteria of the Bacillus cereus group, and wherein said method allows for detection of bacteria of the Bacillus cereus group before the hydrolysis of the PC-PLC substrate by Gram-positive bacteria other than those belonging to the Bacillus cereus group.