Recombinant <i>Flavivirus </i>vaccines

摘要

The invention provides recombinant flavivirus vaccines that can be used in the prevention and treatment of flavivirus infection. The vaccines of the invention contain recombinant flaviviruses including attenuating mutations.

主权项

1. A chimeric flavivirus comprising a yellow fever virus in which the yellow fever virus membrane and envelope proteins have been replaced with the membrane and envelope proteins of a West Nile virus, wherein the West Nile virus envelope protein comprises substitutions in amino acid positions 107, 316, and 440, and the chimeric flavivirus further comprises two or more mutations selected from the group consisting of: (a) a deletion in 3′ untranslated region sequences that comprises less than 30 nucleotides, (b) a deletion in capsid protein sequences, and (c) one or more additional substitutions in the West Nile virus envelope protein at amino acid positions 138, 176, 177, or 280.