Process for production of cadaverine

摘要

By a method for producing cadaverine by culturing a microorganism that extracellularly secretes lysine decarboxylase, by-production of lysine is suppressed, the yield of cadaverine relative to glucose consumption is improved compared to conventional production methods, and further, the load on the purification step in purification of cadaverine as a raw material for polyamide can be reduced.

主权项

1. A method of producing cadaverine comprising:
culturing a transformed microorganism with a gene encoding lysine decarboxylase that extracellularly secretes lysine decarboxylase to increase yield of cadaverine relative to glucose consumption, wherein the microorganism is selected from the group consisting of Corynebacterium glutamicum and E. coli, the microorganism intracellularly expresses a protein comprising lysine decarboxylase having a secretory signal peptide attached to the N-terminus side of the amino acid sequence thereof, which lysine decarboxylase is thereby extracellularly secreted, and the secretory signal peptide is a peptide represented by the amino acid sequence shown in any of SEQ ID NOs: 13 to 43 or a peptide having a sequence identity of not less than 85% to the amino sequence shown in any of SEQ ID NOs: 13 to 43.