| 发明名称 |
Microfluidic device screening method |
| 摘要 |
The invention provides methods of screening a microbial host cell for a property of interest in a microfluidic device, the method comprising the steps of: a) transforming a d-alanine racemase-deficient microbial host cell with a polynucleotide construct comprising: i) one or more polynucleotide region providing the property of interest when present in the host cell, and ii) at least one polynucleotide region complementing the d-alanine racemase deficiency when present in the host cell; and b) screening the transformed host cell for the property of interest in the microfluidic device in the absence of externally provided d-alanine. |
| 申请公布号 |
US8871441(B2) |
申请公布日期 |
2014.10.28 |
| 申请号 |
US200913059454 |
申请日期 |
2009.08.14 |
| 申请人 |
Novozymes A/S |
发明人 |
Lenhard Thomas;Bjoernvad Mads Eskelund;Pedersen Poul Erik |
| 分类号 |
C12Q1/68;C12Q1/02;C12Q1/533;C12N1/21 |
主分类号 |
C12Q1/68 |
| 代理机构 |
|
代理人 |
Lambiris Elias |
| 主权项 |
1. A method of screening a Bacillus host cell for a property of interest in a microfluidic device, the method comprising the steps of:
(a) transforming a d-alanine racemase-deficient Bacillus host cell with a polynucleotide construct comprising:
(i) one or more polynucleotide region(s) providing the property of interest when present in the host cell, and(ii) at least one polynucleotide region complementing the d-alanine racemase deficiency when present in the host cell; and (b) screening the transformed host cell for the property of interest in the microfluidic device in the absence of externally provided d-alanine. |
| 地址 |
Bagsvaerd DK |
