METHOD FOR DOSING THE CONTROL CAPACITY OF C1INH

摘要

The present invention relates to a method for dosing the control capacity of plasma serpin (SERine Protease INhibitor) C1 inhibitor (C1Inh) on the basis of a patient blood sample. The invention also relates to a kit specially designed for said dosing.

主权项

1. A method for dosing the control capacity of the protein C1 plasma inhibitor (C1Inh) based on a sample of plasma from a patient, comprising the following steps:
a) a reaction mixture is prepared based on prekallikrein (pKK), high molecular weight kininogen (HK) and activated Hageman factor protease (FXIIa), the reaction mixture being adjusted in such a manner as to have a pH higher than 7; b) the plasma sample of the patient is incubated with a serine protease inhibitor for a duration at least equal to 5 minutes, in such a manner as to obtain a plasma sample without spontaneous protease activity and in which said inhibitor is inactivated or becomes inactive with regard to the reaction mixture prepared at step a); c) the plasma sample obtained at step b) is incubated with the reaction mixture prepared at step a) for a duration lower than or equal to 20 minutes; d) a chromogenic or fluorogenic substrate of the kallikrein (KK), liable to release a chromophore or a fluorophore after hydrolysis by KK, is added to the plasma sample obtained at step c); e) the possible release of the chromophore or fluorophore obtained at step d) is detected over time; and f) the control capacity of C1Inh is determined based on the detection achieved at step e).