METHODS AND COMPOSITIONS FOR DETECTING THE PRESENCE OF ONE OR MORE AUTOANTIBODIES

摘要

This disclosure describes methods and compositions for immunohistochemically detecting the presence of a SRP autoantibody in a biological sample.

主权项

1. A method of detecting the presence of a SRP autoantibody in a biological sample, comprising the steps of:
contacting a tissue section with a biological sample and a detectably-labeled secondary antibody under conditions in which a complex is formed between SRP polypeptides in the tissue section, and a corresponding SRP autoantibody in the biological sample, if present, and the detectably-labeled secondary antibody; and identifying a pattern of complex formation in the tissue sample by detecting the detectably-labeled secondary antibody, wherein the presence of a pattern of complex formation is indicative of the presence of a SRP autoantibody in the biological sample, and wherein the absence of a pattern of complex formation is indicative of the absence of a SRP autoantibody in the biological sample.