发明名称 |
High Throughput Selection of Specific Cell Binding and Lytic Polypeptides |
摘要 |
The invention provides methods for identifying cell binding and/or lytic polypeptides that permit production of specific polypeptide therapeutics in a high throughput manner. |
申请公布号 |
US2014315751(A1) |
申请公布日期 |
2014.10.23 |
申请号 |
US201214359389 |
申请日期 |
2012.11.01 |
申请人 |
Domenyuk Valeriy;Diehnelt Chris;Johnston Stephen |
发明人 |
Domenyuk Valeriy;Diehnelt Chris;Johnston Stephen |
分类号 |
G01N33/569 |
主分类号 |
G01N33/569 |
代理机构 |
|
代理人 |
|
主权项 |
1. A method for identifying lytic polypeptides, comprising
(a) contacting one or more addressable polypeptide arrays with:
(i) a first population of cells of interest comprising an intracellular detectable marker (IDM); and(ii) a second population of the cells of interest comprising a cell surface detectable marker (CSDM);wherein the addressable polypeptide arrays comprises at least 1000 polypeptides of between 15-24 amino acids in length in defined locations on the array(s), and wherein the contacting occurs under conditions suitable to promote binding of polypeptides to the cells of interest; and (b) detecting binding of the polypeptides to the cells of interest; wherein a polypeptide that binds cells in the first population of cells without damage to the cells and binds cells in the second population of cells without damage to the cells is a binding polypeptide for the cells of interest; and wherein a polypeptide that binds cells in the first population of cells with damage to the cells and binds cells in the second population of cells with damage to the cells is a lytic polypeptide for the cell of interest. |
地址 |
Tempe AZ US |