Protein Chromatography Matrices with Hydrophilic Copolymer Coatings

摘要

A coating of a random copolymer of acrylamide and a second monomer, e.g. glycidoxylmethacrylate, for a silica surface is described. The coating is applied to chromatographic support structures having silica based surfaces. The coating is functionalized to produce protein chromatography matrices that are particularly useful for extracting trace amounts of biomarker molecules from biological samples.

主权项

1. A chromatography matrix comprising
a. a solid support, and b. a surface coating on the solid support, the surface coating comprising a copolymer and a binding ligand, the copolymer including acrylamide and a second monomer including glycidoxylmethacrylate, methacrylate, or 2-(dimethylamino)ethyl methacrylate.