Rapid Enzyme-Linked Immunosorbant Assay for Detection and Identification of Pathogens and Determination of Antimicrobial Susceptibility

摘要

The present invention provides a rapid, highly sensitive and specific enzyme-linked immunosorbent assay (ELISA), referred to as N-Assay, a device, and a kit, for detection and identification of microorganisms in a sample, in thirty minutes or less, with little or no interference from non-target microorganisms. The present invention also provides for simultaneous determination of antimicrobial susceptibility of microorganism in the N-Assay.

主权项

1. A device for rapidly detecting and identifying a microorganism in a sample, comprising:
a plurality of wells in a plate; a coating of an antibody specific for a microorganism deposited on surfaces of the plurality of wells; a wash liquid for preparing one or more serial dilutions of the microorganism, and for washing the plurality of wells, said serial dilutions of the microorganism deposited atop the coating in the plurality of wells; an enzyme-conjugated antibody specific for the microorganism which is deposited atop the serial dilutions of the microorganism; a substrate specific to the enzyme conjugated antibody which is deposited atop the enzyme-conjugated antibody for detecting bound enzyme-conjugated antibody; and a stop solution which is deposited atop the substrate which stops reaction of the substrate with the enzyme-conjugated antibody, wherein the detection and the identification of the microorganism in the sample is accomplished in about 30 minutes or less.