FLUORESCENT CAFFEINE SENSOR AND PORTABLE KIT AND MICROFLUIDICS DEVICE FOR CAFFEINE DETECTION

摘要

The present invention relates to Caffeine Orange, a novel aqueous-phase fluorescence turn-on sensor for caffeine that is structurally based on a BODIPY-scaffold. The present invention further provides for methods of detecting and measuring caffeine in aqueous media. A change in the intensity or visible color of the fluorescence is detectable by either a fluorimeter or by the naked eye. The methods disclosed herein provide for the utilization of a reverse-phase SPE column, optionally as a component in a syringe or a microfluidics-based automation detection system. The invention further provides for the solid phase extraction of an analyte such as caffeine from a liquid medium, the extraction occurring on a microfluidic disc.