NOVEL METHOD

摘要

The present invention relates to a method for degrading host cell nucleic acids associated with a virus or a viral antigen thereof produced by cell culture, the method comprising at least two steps of nucleic acids degradation with a compound selected from i) an endonuclease and ii) a DNA alkylating agent.

主权项

1. A method for producing a virus or a viral antigen thereof in cell culture comprising the steps of:
(a) providing a population of cells cultured in a cell culture medium, (b) inoculating the population of cells with a virus, (c) culturing the population of cells so as to allow the virus to replicate, (d) collecting the produced virus thereby providing a viral harvest, and (e) isolating the virus,the method comprising at least one step of host cell nucleic acids degradation with an endonuclease and at least one step of host cell nucleic acids degradation with a DNA alkylating agent.