IMAGING MASS ANALYSIS DATA PROCESSING METHOD AND IMAGING MASS SPECTROMETER

摘要

If spatial measurement point intervals in imaging mass analysis data of two samples to be compared are different and the degrees of spatial distribution spreading of substances are compared, one of the data is defined as a reference, the measurement point intervals in the other of the data are redefined so as to be equalized to the reference, and a mass spectrum at each virtual measurement point set as a result of the redefinition is obtained through interpolation or extrapolation based on a mass spectrum at an actual measurement points. If the arrays of the m/z values of mass spectra are different for each sample, the m/z value positions of the mass spectrum in one of the data are defined as a reference, and the intensity values corresponding to the reference m/z values are obtained through interpolation or extrapolation for the mass spectrum of the other of the data.

主权项

1. An imaging mass analysis data processing method of processing imaging mass analysis data in which mass spectrum data collected by performing a mass analysis on each of a plurality of measurement points on a sample is associated with spatial position information of the measurement point, comprising:
a) a spatial correction step in which a spatial measurement point interval in one of a plurality of imaging mass analysis data is defined as a reference, and mass spectrum data at each of virtual measurement point positions in other imaging mass analysis data is obtained through interpolation or extrapolation using mass spectrum data at a plurality of measurement points around the virtual measurement point position, the virtual measurement point positions being virtual positions in the other imaging mass analysis data if a measurement point interval is equalized to the reference; b) a mass-to-charge ratio correction step in which a mass-to-charge ratio range common to mass spectra in the plurality of imaging mass analysis data is extracted, mass-to-charge ratio points within the extracted common mass-to-charge ratio range in one of the plurality of imaging mass analysis data are defined as references, and an intensity value at each of virtual mass-to-charge ratio points in the other imaging mass analysis data is obtained through interpolation or extrapolation using intensity values at actually measured mass-to-charge ratio points before and after the virtual mass-to-charge ratio point, the virtual mass-to-charge ratio points in the other imaging mass analysis data being mass-to-charge ratio points if mass-to-charge ratio points in the other imaging mass analysis data are equalized to the references; and c) a combining step, in which the plurality of imaging mass analysis data in which the measurement point intervals and the mass-to-charge ratio points are equalized by performing the spatial correction step and the mass-to-charge ratio correction step are combined so as to be treatable as one imaging mass analysis data.