DNA fragment and use thereof

摘要

The invention relates to a DNA consisting of the nucleotide sequence shown in SEQ ID NO: 1, or a nucleotide sequence the same or substantially the same as a partial nucleotide sequence thereof containing at least one HRE consensus sequence, which transiently has a transcription promoting activity in a vascular endothelial cell-specific manner in a hypoxic state, or a vector containing a promoter containing the DNA, and the like. The prophylaxis and/or treatment, as well as diagnosis, of acute ischemic diseases are enabled by connecting a prophylactic and/or therapeutic gene or a reporter gene to the downstream of the vector and administering same to mammals.

主权项

1. A method for promoting vascular endothelial cell-specific transcription of a gene in cells in an acute ischemic stage in a patient, which comprises administrating to the patient by catheter or injection an expression vector comprising a gene operably linked to a promoter such that an effective amount of the expression vector is delivered to the cells in an acute ischemic stage, wherein the promoter comprises a nucleotide sequence having a sequence identity of not less than 80% to (a) the nucleotide sequence of SEQ ID NO: 1 or (b) the nucleotide sequence of a non-human mammalian ortholog of the nucleotide sequence of SEQ ID NO: 1, wherein the non-human mammalian ortholog is a mouse or rat ortholog, and wherein the gene operably linked to the promoter is transiently expressed in the cells in an acute ischemic stage in the patient in a vascular endothelial cell-specific manner, thereby promoting vascular endothelial cell-specific transcription of the gene in the cells in an acute ischemic stage in the patient.