Simplified and improved method for preparing an antibody or an antibody fragment targeted immunoliposome for systemic administration of a therapeutic or diagnostic agent

摘要

Provided herein is an antibody- or antibody fragment-targeted cationic immunoliposome complex prepared by a method comprising the steps of (a) preparing an antibody or antibody fragment; (b) mixing the antibody or antibody fragment with a cationic liposome and to form a cationic immunoliposome wherein the antibody or antibody fragment is complexed with the cationic liposome, but is not chemically conjugated to the cationic liposome and wherein said antibody or antibody fragment does not comprise a lipid tag; and (c) mixing the cationic immunoliposome with a therapeutic or diagnostic agent to form the antibody- or antibody fragment-targeted cationic immunoliposome. Also provided is an antibody- or antibody fragment-targeted cationic immunoliposome comprising an antibody or antibody fragment; a cationic liposome, wherein the antibody or antibody fragment is complexed with the cationic liposome, but is not chemically conjugated to the cationic liposome and wherein said antibody or antibody fragment does not comprise a lipid tag and a therapeutic or diagnostic agent.

主权项

1. An antibody- or antibody fragment-targeted cationic immunoliposome complex comprising:
a) an antibody or antibody fragment; b) a cationic liposome, wherein said antibody or antibody fragment is complexed with said cationic liposome, but is not chemically conjugated to said cationic liposome, and wherein said antibody or antibody fragment does not comprise a lipid tag; and c) an agent selected from the group consiting of a therapeutic agent, a diagnostic agent and a reporter gene,wherein said immunoliposome complex is 50-400 nm in size.