Protein production method, fusion protein, and antiserum

摘要

Disclosed are a highly efficient method for production of heterologous proteins performed by utilizing microorganisms, as well as fusion proteins, and an antiserum. The method includes a method for production of a protein (A) in the form of a fusion protein, comprising the steps of (a) preparing a DNA which codes for a fusion protein comprising the peptide chain forming the protein (A) and the C-terminal peptide or its fragment (B) of the Cry proteins produced by Bacillus thuringiensis, and (b) introducing the DNA into a host bacterium to transform the same, and (c) allowing the fusion protein to be expressed in the transformed host bacterium, as well as a method for production of the protein (A) itself comprising a further step of removing the peptide chain (B) from the fusion protein obtained.

主权项

1. A method for production of a protein (A) in the form of a fusion protein, comprising the steps of
(a) preparing a DNA which codes for a fusion protein comprising the peptide chain forming the protein (A) and a second peptide chain (B), wherein the N-terminus of protein (A) is fused to the C-terminus of peptide (B) either directly or via a spacer; or the C-terminus of protein (A) is fused to the N-terminus of peptide (B) either directly or via a spacer, wherein the peptide chain (B) consists of a part of the amino acid sequence that starts with Ile696 and ends with Glu1180 of SEQ ID NO: 89, and the part comprises the sequence set forth in SEQ ID NO: 6, (b) introducing the DNA into a host bacterium, and (c) expressing the fusion protein in the host bacterium.