发明名称 |
Method for controlled release of parathyroid hormone from encapsulated poly(lactic-glycolic)acid microspheres |
摘要 |
The present invention provides a method for producing a controlled release microsphere with mean average size greater than 50 μm, comprising preparing a water-in-oil (w/o) emulsion comprising an inner aqueous layer containing a pharmaceutically effective amount of a biologically active polypeptide with activity similar to parathyroid hormone, and an oil layer containing a polymer substance of poly(lactic-co-glycolic acid) (PLGA), then adding the w/o emulsion into aqueous polyvinyl alcohol (PVA) solution to form a water-in-oil-in-water (w/o/w) double emulsion and then desorbing the solvent in the oil layer. The present invention also provides a controlled release microsphere prepared by the method and use thereof. |
申请公布号 |
US8865220(B2) |
申请公布日期 |
2014.10.21 |
申请号 |
US201012814955 |
申请日期 |
2010.06.14 |
申请人 |
Kaohsiung Medical University |
发明人 |
Ho Mei-Ling;Wang Gwo-Jaw;Chang Je-Ken;Fu Yin-Chih;Tzeng Cherng-Chyi;Rajalakshmanan Eswaramoorthy |
分类号 |
A61K9/16;A61K9/50;A61K38/29 |
主分类号 |
A61K9/16 |
代理机构 |
WPAT, P.C. |
代理人 |
WPAT, P.C. ;King Anthony |
主权项 |
1. A method for controlled release delivery of biologically active polypeptide with activity similar to parathyroid hormone to a subject suffering from osteoarthritis in effective therapeutic concentration comprising: administrating to the subject a controlled release microsphere with mean average size greater than 50 μm, which is produced by preparing a water-in-oil (w/o) emulsion comprising an inner aqueous layer containing the biologically active polypeptide, and an oil layer containing a polymer substance of poly(lactic-co-glycolic acid) (PLGA), then gradually adding the w/o emulsion into aqueous polyvinyl alcohol (PVA) solution to form a water-in-oil-in-water (w/o/w) double emulsion and then desorbing the solvent in the oil layer, whereby the polypeptide is released in the effective therapeutic concentration ranging from about 1×10−7 M to about 5×10−9 M for at least 18 days. |
地址 |
Kaohsiung TW |