Fluorescent chemical compounds having high selectivity for double stranded DNA, and methods for their use

摘要

Chemical compounds having a high selectivity for double stranded DNA over RNA and single stranded DNA are disclosed. The chemical compounds are stains that become fluorescent upon illumination and interaction with double stranded DNA, but exhibit reduced or no fluorescence in the absence of double stranded DNA. The compounds can be used in a variety of biological applications to qualitatively or quantitatively assay DNA, even in the presence of RNA.

主权项

1. A chemical compound having the structure: wherein: n is 0; X is oxygen; R1, R2, R3, R4, R5, R6, R7, R8, R10, and R11 independently comprise hydrogen, a hydroxyl group, an alkoxy group, a thiol, a thioalkyl, a thioaryl, a halogen, an alkyl group, an alkenyl group, an alkynyl group, an aromatic group, a primary amine group, a secondary amine group, a tertiary amine group, a reactive group, or combinations thereof, wherein at least one of R1, R2, R3, and R4 comprises an aromatic group or an alkynyl group; R9 comprises an aromatic group or alkyl-aromatic group; and R12 is an alkyl group.