Nucleic acid analysis device and nucleic acid analyzer using the same

摘要

The present invention relates to a nucleic acid analysis device for analysis of nucleic acid in a sample through fluorometry, in which a localized surface plasmon by light irradiation, and in which a nucleic acid probe or a nucleic acid synthase for the analysis of the nucleic acid in the sample is disposed in a region of generation of the surface plasmon. The present invention allows the fluorescence intensifying effect of the surface plasmon to be produced efficiently and also enables the immobilization of a DNA probe or the nucleic acid synthase in a region on which the fluorescence intensifying effect is exerted, thus making it possible to carry out a measurement on the base elongation reaction without having to remove the unreacted substrate with the fluorescent molecule.

主权项

1. A nucleic acid analysis device for analysis of nucleic acid in a sample through fluorometry, comprising:
a substrate comprising a plurality of metal structures disposed thereon, wherein each of the plurality of metal structures (a) forms a projection above the substrate (b) has a base attached to the substrate and an apex extending above the substrate and (c) consists of one or more metals in which a localized surface plasmon is generated by light irradiation; an insulting layer, wherein the insulating layer is disposed above the substrate and around the plurality of metal structures in a manner that the apex of said each of the plurality of metal structures is exposed and not covered by the insulating layer; a nucleic acid synthase immobilized on the exposed surface of said each of the plurality of metal structures; and a nucleic acid probe bound to the nucleic acid synthase for the analysis of the nucleic acid in the sample, wherein one molecule of the nucleic acid probe is bound to each nucleic acid synthase, and the nucleic acid synthase is disposed in a region on which the localized surface plasmon is generated.