RECOMBINANT PLASMID DNA PET3.54, ENCODING POLYPEPTIDE FN3.54, INTERACTING WITH HUMAN TUMOUR NECROSIS FACTOR, AND STRAIN OF BACTERIA ESCHERICHIA COLI - PRODUCER OF POLYPEPTIDE FN3.54, INTERACTING WITH HUMAN TUMOUR NECROSIS FACTOR

摘要

FIELD: biotechnology.SUBSTANCE: invention is a recombinant plasmid DNA pET3.54, encoding the polypeptide FN3.54, interacting with human tumour necrosis factor. The present invention also discloses the recombinant strain of bacteria Escherichia coli BL21(DE3)/pET3.54 - producer of the polypeptide FN3.54, interacting with human tumour necrosis factor.EFFECT: invention enables to obtain the desired protein with high yield, using a smaller amount of the inducer and the expression level of total cell protein.2 cl, 4 dwg, 6 ex