| 摘要 |
The present invention relates to a doubly mutated BKCa channel construct and a novel cell-based screening system for ion channel modulators using same. A doubly mutated BKCa channel-comprising cell-based system of the present invention, when compared to a control group, shows remarkably increased fluorescence caused by membrane depolarization, regardless of whether there is a separate increase of [Ca2+]i, and shows activity (movement in the negative direction of a G/V curve) that is further triggered by a known activator (for example, CTBIC). Therefore, compared to conventional methods (for example, an automated patch clamping method), the system of the present invention is capable of more effectively and far more accurately analyzing the activity of an ion channel, and thus can be effectively applied not only to the separation/identification of ion channel modulators but also to a screening for a therapeutic agent for a condition, disease or disorder related to the modulation of a BKCa channel. |
