METHOD FOR QUANTITATIVELY SENSING AND EFFECTIVELY MARKING INTERACTION WITH TARGET MATERIAL BY USING ENERGY TRANSFER AND SIGNAL CHANGE BASED ON HIGH-DENSITY DISPLAY OF MATERIAL

摘要

The present invention relates to methods of quantitatively analyzing and detecting interactions, which effectively occur between detector materials displayed on a nano-assembly matrix at high density, and sensitively labeling and isolating a target material that effectively interacts with the detector materials, by the use of energy transfer and signal changes that effectively occur in label molecules displayed at high density. Specifically, the present invention relates to a method of quantitatively detecting the interactions between various bioactive materials, which occur in vitro or in vivo, based on high-density displays of molecules, and to a method of sensitively labeling a target material that interacts with these bioactive materials.

主权项

1. A method for labeling or isolating a target material interacting with a bait, the method comprising the steps of:
(i) providing mediator (regulator) materials, bait materials and nano-assembly matrix-forming materials to the same field or system; (ii) forming a nano-assembly unit matrix and/or nano-assembly matrix by the interaction between the mediator (regulator) materials; the mediator (regulator) materials and the bait materials; the bait materials; the nano-assembly matrix-forming materials; the mediator (regulator) materials and the nano-assembly forming materials; and/or the bait materials and the nano-assembly forming materials to display the bait materials at high density; (iii) providing a library of prey materials to the nano-assembly unit matrix and/or nano-assembly matrix; (iv) labeling and selecting a prey material as the target material by interaction with the bait materials displayed on the nano-assembly unit matrix and/or nano-assembly matrix at high density; and (iv) confirming the labeled target material using the energy transfer or signal change, or isolating and identifying the confirmed target material, wherein at least one of the mediator (regulator) material, the bait material, the nano-assembly material-forming material and the prey material is labeled with a label.