Methods of Screening for Inhibitors of Enzymes

摘要

Methods and compositions for detection of the modulators of proteolytic enzymes, particularly cysteine proteases, are disclosed.

主权项

1. A method for screening for modulators of an enzyme wherein said enzyme comprises an active site cysteine, said method comprising measuring the activity of at least one active site mutant of said enzyme in the presence of at least one compound,
wherein a modulation in the activity of the mutant enzyme in the presence of the compound compared to the activity of the mutant enzyme in the absence of the compound indicates that the compound is a modulator of the wild-type enzyme.