| 摘要 |
<p>A method for quantifying a nucleic acid in an original sample wherein the nucleic acids in the original sample are tagged with oligonucleotides to form a plurality of labelled-nucleic acid molecules, the treating the sample with a uracil DNA glycosylase (UDG), amplifying these treated labelled nucleic acid molecules and quantifying a nucleic acid molecule in the sample. The method may produce a plurality of labelled amplicons with a unique tag identifier regions. Alternatively claimed is a clonal amplification method comprising stochastically labelling a plurality of molecules with a plurality of oligonucleotide tags, wherein the plurality of molecules comprises at least two different sequences, the plurality of tags comprises at least 2 different oligonucleotide sequences, the labelled molecules are amplified to produce labelled amplicons, the labelled amplicons are amplified by nested PCR to produce a plurality of nested PCR amplified labelled amplicons and detecting said plurality of nested PCR labelled amplicons.</p> |
