Method for the production of a strain having a deleted region in chromosome

摘要

The purpose of the present invention is therefore to provide a transtormani which will not produce a toxic substance such as Aflatoxin even after being manipulated with genetic engineering by efficiently deleting a large chromosomal region with a length of from several tens to hundreds kb such as a cluster of biosynthesis genes encoding the toxic substances such as Aflatoxin. The present invention is related to a method for the production of a strain having a deleted region in chromosome using a transformant having an increased frequency of homologous recombination due to suppression of a Ku gene , which is a mitosporic filamentous fungus belonging to Trichocomaceae, comprising transforming said transformant so as to include a homologous region in both ends of a chromosomal region to be deleted, and deleting the chromosomal region by means of homologous recombination based on said homologous region.