Simultaneous, integrated selection and evolution of antibody/protein performance and expression in production hosts

摘要

The present disclosure provides methods of integrating therapeutic protein and antibody generation and/or selection, evolution and expression in a eukaryotic host for manufacturing in a single system. Therapeutic proteins, including antibodies, are generated, optimized and manufactured in the same eukaryotic host system. The disclosed system of Comprehensive Integrated Antibody Optimization (CIAO!™) allows for simultaneous evolution of protein performance and expression optimization.

主权项

1. A method of evolution and manufacture of an antibody in a CHO cell production host; the method comprising the steps of:
a. selecting a full-length template antibody; b. evolving the template antibody to enable production of a set of mutant antibodies in the CHO cell production host; c. screening the mutant antibodies for at least one predetermined property, characteristic or activity; d. selecting an up-mutant antibody from the set of mutant antibodies based upon optimization of the at least one predetermined property, characteristic or activity as compared to a same property, characteristic or activity of the template antibody; and e. manufacturing the up-mutant antibody in the CHO cell production host.