Manufacture of chromatography matrices

摘要

The present invention relates to a method of manufacturing a chromatography matrix comprising providing a polysaccharide carrier comprising available hydroxyl groups; and reacting said hydroxyl groups with vinyl sulphonate to provide a sulphonate-functionalized (S-functionalized) cation exchanger. The hydroxyl groups of the carrier may be hydroxyls of the agarose polymer; or alternatively they may be provided on extenders such as polyhydroxyfunctional polymers. In one embodiment, the carrier is made of agarose with improved flow pressure properties.

主权项

1. A method of manufacturing a chromatography matrix comprising
providing at least one cross-linked agarose carrier comprising available hydroxyl groups; and reacting said hydroxyl groups with vinyl sulphonate to provide a sulphonate-functionalized (S-functionalized) cation exchanger; wherein the hydroxyl groups are coupled to the carrier surface via extenders that comprise dextran; further wherein the carrier is provided by: (a) providing an aqueous agarose solution and substituting part of the hydroxyl groups of said agarose; (b) gelling the agarose solution to provide a carrier; and (c) cross-linking the agarose gel obtained from (b) by reacting hydroxyl groups of the agarose.