Apparatus and methods for analysing fluorescent particles

摘要

According to an embodiment of the invention, an apparatus to detect fluorescence from a sample is disclosed. The apparatus comprises a sample plane onto which the sample is arranged, an excitation light unit including at least a light source to illuminate the sample, and a detection unit comprising at least a detector having at least 100,000 active detection elements to detect a fluorescence signal from the sample.

主权项

1. A method for detecting viability of a test cell, said method comprising:
mixing the test cell with a combination of 4,6-diamion-2-phenylindole (DAPI) dye or substituted variance of DAPI and Acridine Orange or other stains of Acridine; allowing staining of cells for a time period of at the most 60 minutes, such as 30 minutes, such as 20 minutes, such as at most 10 minutes; obtaining a stained cell sample, wherein the Acridine Orange/other stains of Acridine stains the entire population of cells and DAPI/substituted variance of DAPI stains only non-viable cell; exposing the stained test cell to an excitation light; detecting a fluorescence signal from the stained test cell; and processing the fluorescence signal to identify whether the test cell is non-viable.