Method for producing cells having characteristics of hepatocytes

摘要

The present invention relates to a method of producing cells having at least one characteristic of human hepatocytes as well as to cells produced by said method and uses of these cells.

主权项

1. A method for producing cells having at least one characteristic of human hepatocytes, said method comprising the steps of:
(a) seeding human monocytes in a culture vessel in a cell culture medium; (b) culturing the monocytes in a cell culture medium comprising interleukin 3 (IL-3) and adenosine; and (c) culturing the cells subsequent to step (b) in a cell culture medium comprising fibroblast growth factor 4 (FGF-4), wherein the cell culture medium comprising FGF-4 does not comprise adenosine.