Integrated calibration sample bay for fluorescence readers

摘要

A stage assembly, an imaging system that uses the stage assembly, and methods for using the stage assembly in a high content screening system. The stage assembly includes a stage having a top surface and an opposing bottom surface and an opening extending between the top and bottom surfaces to receive a specimen plate. The stage assembly also includes a calibration sample bay formed in the stage. A calibration sample can also be secured within the calibration sample bay.

主权项

1. A stage assembly for use in a high content screening system, the stage assembly comprising:
a stage having a top surface and an opposing bottom surface, the stage bounding an opening extending between the top and bottom surfaces; a specimen plate holder removably positioned within the opening, the specimen plate holder being configured to receive a microtiter plate holding a plurality of biological cells; a calibration sample bay formed on the stage at a location spaced apart from the opening; and a calibration sample removably secured within the calibration sample bay, the calibration sample being comprised of a fluorescent material.