Affinity chromatography matrix

摘要

The present invention relates to a method of separating one or more immunoglobulin containing proteins from a liquid. The method includes first contacting the liquid with a separation matrix comprising ligands immobilised to a support; allowing the immunoglobulin containing proteins to adsorb to the matrix by interaction with the ligands; followed by an optional step of washing the adsorbed immunoglobulin containing proteins; and recovering said immunoglobulin containing proteins by contacting the matrix with an eluent which releases the proteins. The method improves upon previous separation methods in that each of the ligands comprises one or more of a protein A domain (E, D, A, B, C), or protein Z, or a functional variant thereof, with at least one of the monomers having a substitution of the C-terminal most proline residue after the third alpha-helix.

主权项

1. A ligand for separation of immunoglobulin containing proteins, which ligand comprises one or more monomeric domains of staphylococcal protein A (SpA) (E, D, A, B, C) or protein Z, wherein the proline nearest the C terminus in at least one of the domains has been substituted with any other amino acid.